Cooperation between International Burch University and BiOptimizers - International Burch University
Biofilms
September 2, 2020
Serdar Dayan Ph.D Disertation Defense
September 4, 2020

Cooperation between International Burch University and BiOptimizers

Consumers are becoming increasingly conscientious of what they eat and there is a growing awareness regarding positive effects of food outside of nutritional value (1). Probiotics are ‘good’ microorganisms that cannot sedate hunger nor provide many calories; however, they provide other benefits. These include elimination of harmful bacteria, controlling the existing microflora within your system, and stabilizing gut and immune health due to the abilities of good bacteria (2). 

A cooperation between International Burch University (IBU) and BiOptimizers, an innovative, international supplement company focusing on probiotics and overall health, has been established earlier this year, whereby personnel from the Department of Genetics and Bioengineering at IBU have been analyzing the properties of numerous products from BiOptimizers’ product range.

From the BiOptimizers’ product range, Leaky Gut Guardian (vanilla and chocolate flavored), P3OM and CogniBiotics are four popular probiotic products currently being sold across the globe, and are being analyzed for their probiotic potential and antibiotic properties via a range of different experiments. 

Leaky Gut Guardian contains prebiotics (products that induce growth of probiotics) and probiotics, which are used to repair compromised gut-lining, improve gut health, raise energy levels, and other factors. P3OM is a single patented probiotic strain, meaning it only contains one type of bacteria, that claims to optimize gut health, burn fat, and build muscles. CogniBiotics is a breakthrough formula with numerous probiotic strains used to boost low moods, reduce stress, and support brain activity.

According to initial analysis, the four products have provided noteworthy results in their abilities to tolerate environmental stresses such as different acid and salt concentrations. This is crucial as probiotics must be able to climatize to the host, specially the turbulent nature of the gut, to confer health benefits (3). Remarkably, P3OM survived and thrived in highly saline solutions, whereas other referent lactic acid bacteria strains could not. 

The four products have also proven successful under numerous safety assays, which are a series of experiments that confirm that the host will not be harmed by the product. This is shown by the product being resistant or slightly sensitive to commonly administered antibiotics, which is optimal for maintenance of host gut microbial flora as ‘good’ bacteria will not be compromised by biocidal products (4). Additionally, in terms of safety assays, the products lack hemolytic activity meaning the probiotic bacteria are unable to lyse red blood cells, which is a pathogenic, or ‘bad’ bacteria, tendency.  

Investigation of antimicrobial activity has revealed the products’ ability in inhibiting growth of gut specific pathogens such as Escherichia coli, and other opportunistic pathogens such as Staphylococcus aureus, Enterococcus faecalis and Bacillus subtilis subsp. spizizenii. Other antimicrobial tests have found that all four products can stabilize and even aid in proliferation of beneficial bacteria (such as Pediococcus acidilactici, Bifidobacterium longum and a number of Lactobacillus plantarum strains), which are crucial in retaining gut health (5).   

Future experiments will lead towards the study of biofilm formation, testing against other hormetic stresses (such as sound wave stress or hormone stress), aggregation assays (their ability to bind to the host’s gut wall) and virulence activity.

The results are looking very promising for these four products!

 REFERENCES

  1. Kechagia, M., Basoulis, D., Konstantopoulou, S., Dimitriadi, D., Gyftopoulou, K., Skarmoutsou, N., & Fakiri, E. M. (2013). Health Benefits of Probiotics: A Review. ISRN Nutrition, 2013, 1–7. https://doi.org/10.5402/2013/481651
  2. Gómez, N. C., Ramiro, J. M. P., Quecan, B. X. V., & de Melo Franco, B. D. G. (2016). Use of Potential Probiotic Lactic Acid Bacteria (LAB) Biofilms for the Control of Listeria monocytogenes, Salmonella Typhimurium, and Escherichia coli O157:H7 Biofilms Formation. Frontiers in Microbiology, 7. https://doi.org/10.3389/fmicb.2016.00863
  3. Bin Masalam, M. S., Bahieldin, A., Alharbi, M. G., Al-Masaudi, S., Al-Jaouni, S. K., Harakeh, S. M., & Al-Hindi, R. R. (2018). Isolation, Molecular Characterization and Probiotic Potential of Lactic Acid Bacteria in Saudi Raw and Fermented Milk. Evidence-Based Complementary and Alternative Medicine, 2018, 1–12. https://doi.org/10.1155/2018/7970463
  4. Chen, C.-C., Lai, C.-C., Huang, H.-L., Huang, W.-Y., Toh, H.-S., Weng, T.-C., Chuang, Y.-C., Lu, Y.-C., & Tang, H.-J. (2019). Antimicrobial Activity of Lactobacillus Species Against Carbapenem-Resistant Enterobacteriaceae. Frontiers in Microbiology, 10, 789. https://doi.org/10.3389/fmicb.2019.00789
  5. Papadimitriou, K., Zoumpopoulou, G., Folign, B., Alexandraki, V., Kazou, M., Pot, B., & Tsakalidou, E. (2015). Discovering probiotic microorganisms: In vitro, in vivo, genetic and omics approaches. Frontiers in Microbiology, 6. https://doi.org/10.3389/fmicb.2015.00058